abstract：:The olfactory epithelium (OE) of the mouse provides a unique system for understanding how cell birth and cell death interact to regulate neuron number during development and regeneration. We have examined cell death in the OE in normal adult mice; in adult mice subjected to unilateral olfactory bulbectomy (surgical re...

journal_title：Developmental biology

authors： Holcomb JD,Mumm JS,Calof AL

更新日期：1995-11-01 00:00:00

abstract：:Gastrulation in Xenopus laevis transforms a ball of undifferentiated cells into a well-organized elongated embryo with a distinct body axis, as a result of extensive cell rearrangements and movements. Since cell adhesion is thought to play an important role during tissue morphogenesis, we investigated the potential ro...

journal_title：Developmental biology

authors： Lee CH,Gumbiner BM

更新日期：1995-10-01 00:00:00

abstract：:In the marine bivalve Hiatella flaccida, full-grown oocytes in ovaries are arrested at the first prophase (prophase-I) of meiosis, whereas spawned oocytes have reinitiated meiosis from prophase-I and are again arrested at the first metaphase (metaphase-I). The neurohormone serotonin (5-hydroxytryptamine, 5-HT) was abl...

journal_title：Developmental biology

authors： Deguchi R,Osanai K

更新日期：1995-10-01 00:00:00

abstract：:In previous studies we found that two glycoproteins of 220 and 180 kDa account for 80-90% of the material dissolved from the vitelline coat (VC) of Unio elongatulus egg (Focarelli and Rosati, 1993). The two glycoproteins were purified by electroelution and used to raise the corresponding polyclonal antibodies. Immunof...

journal_title：Developmental biology

authors： Focarelli R,Rosati F

更新日期：1995-10-01 00:00:00

abstract：:In this study, we present evidence that neurogenic cells inhibit the differentiation of cardiogenic cells. When cells of the entire area pellucida at stage 5 were dissociated and reaggregated, the aggregates differentiated into neural tissues and other structures of any germ layer origin, except for heart tissues, des...

journal_title：Developmental biology

authors： Climent S,Sarasa M,Villar JM,Murillo-Ferrol NL

更新日期：1995-09-01 00:00:00

abstract：:In neural plate stage Xenopus embryos, XlHbox 8 expression marks anterior endodermal cells fated to develop into pancreas/duodenum, and expression continues in adult pancreas in exocrine duct, acinar, and islet cells. Here, XlHbox 8 is used as a marker in experiments addressing the mechanisms of early endodermal patte...

journal_title：Developmental biology

authors： Gamer LW,Wright CV

更新日期：1995-09-01 00:00:00

abstract：:Pulses of ecdysteroids induce dramatic changes in gene expression that direct the early stages of Drosophila metamorphosis. This gene activity is reflected by the appearance of early and late puffs in the salivary gland polytene chromosomes. Curiously, the early puff genes that have been studied to date are induced by...

journal_title：Developmental biology

authors： Baehrecke EH,Thummel CS

更新日期：1995-09-01 00:00:00

abstract：:Mesodermal cell migration during amphibian gastrulation is dependent on cellular interactions with fibronectin. One mechanism whereby cells bind fibronectin is through alpha v-containing integrin heterodimers. In order to investigate the role of alpha v in amphibian gastrulation, we have cloned the Pleurodeles homolog...

journal_title：Developmental biology

authors： Alfandari D,Whittaker CA,DeSimone DW,Darribère T

更新日期：1995-08-01 00:00:00

abstract：:To examine the conditions of cell composition necessary for formation of a complete tadpole, defect embryos of 35 series were prepared by removing particular cells from 16- and 32-cell embryos of Xenopus laevis. These defect embryos were cultured and their development was examined. Formation of a complete tadpole did ...

journal_title：Developmental biology

authors： Kageura H

更新日期：1995-08-01 00:00:00

abstract：:Treatment of metaphase II-arrested hamster eggs with activators of protein kinase C has been reported to promote resumption of the cell cycle, second polar body emission, and pronucleus formation (G.I. Gallicano, S.M. Schwarz, R.W. McGaughey, and D.G. Capco, 1993, Dev. Biol. 156, 94-106). In contrast, we have not obse...

journal_title：Developmental biology

authors： Moore GD,Kopf GS,Schultz RM

更新日期：1995-08-01 00:00:00

abstract：:Proper spatial expression of the wingless (wg) gene in the Drosophila embryonic epidermis is crucial to intrasegmental patterning. Single cell wide wg expression is initiated at the blastoderm stage in response to combinatorial regulation by the pair rule genes. Later, during gastrulation, when the epidermal expressio...

journal_title：Developmental biology

authors： Yoffe KB,Manoukian AS,Wilder EL,Brand AH,Perrimon N

更新日期：1995-08-01 00:00:00

abstract：:In order to disclose the mechanism that regulate synapse development we compared the distributions of agrin, acetylcholine receptors (AChR) and a basal lamina heparan sulfate proteoglycan (HSPG) in sections and cultures prepared from Xenopus laevis and Ambystoma mexicanum embryos. While agrin, AChR and HSPG may accumu...

journal_title：Developmental biology

authors： Anderson MJ,Shi ZQ,Grawel R,Zackson SL

更新日期：1995-07-01 00:00:00

abstract：:Neuropilin (previously known as the A5 protein) is a membrane protein identified in Xenopus and is presumed to be involved in the target recognition of the optic nerve fibers. We have isolated cDNAs encoding the chick homologue of neuropilin, using the Xenopus neuropilin cDNA as a hybridization probe. The predicted am...

journal_title：Developmental biology

authors： Takagi S,Kasuya Y,Shimizu M,Matsuura T,Tsuboi M,Kawakami A,Fujisawa H

更新日期：1995-07-01 00:00:00

abstract：:The transition from maternal to zygotic gene expression in the mouse occurs in the 2-cell embryo. Previous studies in which DNA was injected into 2-cell embryos revealed that transcription promoters and origins of DNA replication are strongly repressed in cleavage stage embryos unless linked to an embryo-responsive en...

journal_title：Developmental biology

authors： Henery CC,Miranda M,Wiekowski M,Wilmut I,DePamphilis ML

更新日期：1995-06-01 00:00:00

abstract：:Development of the Drosophila embryonic midgut is dependent on a number of genes, including tinman and bagpipe, which are required for formation of the visceral mesoderm, and the homeotic genes and their targets, which act locally in the visceral mesoderm to direct formation of specific midgut constrictions. Here we r...

journal_title：Developmental biology

authors： Cimbora DM,Sakonju S

更新日期：1995-06-01 00:00:00

abstract：:Several Strongylocentrotus purpuratus gene cis-regulatory regions contain asymmetric C4 sequences which are core elements of target sites for a specific DNA-protein interaction. Blastula stage nuclear extract contains five proteins which specifically bind to these target sites, resulting in a characteristic pattern of...

journal_title：Developmental biology

authors： Zeller RW,Coffman JA,Harrington MG,Britten RJ,Davidson EH

更新日期：1995-06-01 00:00:00

abstract：:Kidney development is a complex, little understood process based on inductive interactions and intricate epithelial and mesenchymal morphogenesis. Here, we report the use of subtractive hybridization to clone cDNAs expressed in early nephrogenesis. cDNA made from E14.5 mouse kidney was hybridized with adult mouse live...

journal_title：Developmental biology

authors： McConnell JE,Armstrong JF,Hodges PE,Bard JB

更新日期：1995-05-01 00:00:00

abstract：:Cadherin-11 (cad-11) is a novel member of the cadherin family of cell adhesion molecules, having recently been identified by means of the polymerase chain reaction. To study the function and expression of this molecule, we cloned mouse, cad-11 cDNA. Transfection of L cells with cDNA led them to acquire a typical cadhe...

journal_title：Developmental biology

authors： Kimura Y,Matsunami H,Inoue T,Shimamura K,Uchida N,Ueno T,Miyazaki T,Takeichi M

更新日期：1995-05-01 00:00:00

abstract：:The levels of al-1 and al-2 transcripts change dramatically in response to light and development during the formation of Neurospora crassa asexual spores (conidia). al-1 and al-2 mRNAs accumulate throughout conidiation irrespective of lighting conditions initially at low levels. As conidiation proceeds, two increases ...

journal_title：Developmental biology

authors： Li C,Schmidhauser TJ

更新日期：1995-05-01 00:00:00

abstract：:We studied the pathway of 5S ribosomal RNA (rRNA) during oogenesis in Xenopus from its storage in the cytoplasm to incorporation into ribosomes in the nucleus. Ribonucleoprotein particle (RNP) assembly assays reveal striking differences in the behavior of oocyte-type and somatic-type 5S rRNA after microinjection into ...

journal_title：Developmental biology

authors： Allison LA,North MT,Neville LA

更新日期：1995-04-01 00:00:00

abstract：:Syndecan-3 is one of four identified members of a family of transmembrane proteoglycans (the syndecans) that possess highly similar cytoplasmic and transmembrane domains and may function as extracellular matrix receptors and/or low affinity receptors for signaling molecules such as FGF. We previously reported the clon...

journal_title：Developmental biology

authors： Gould SE,Upholt WB,Kosher RA

更新日期：1995-04-01 00:00:00

abstract：:Among the derivatives of the cephalic neural crest is the ectomesenchyme which subsequently constitutes most of the craniofacial skeleton. There is evidence to suggest that the skeletogenic fate of the hindbrain neural crest is specified before emigration from the neural tube and that Antennapedia class Hox genes are ...

journal_title：Developmental biology

authors： Hunt P,Ferretti P,Krumlauf R,Thorogood P

更新日期：1995-04-01 00:00:00

abstract：:We have investigated the imprinting status of two insulin genes using an interspecific recombinant congenic mouse strain carrying Ins1 and Ins2 alleles from Mus spretus on a C57BL/6 genetic background. At Days 12.5, 13.5, and 14.5 of gestation, expression of both parental alleles of both Ins1 and Ins2 was detected in ...

journal_title：Developmental biology

authors： Deltour L,Montagutelli X,Guenet JL,Jami J,Páldi A

更新日期：1995-04-01 00:00:00

abstract：:A new monoclonal antibody, specific to an epitope in the carboxyl terminus of the Drosophila collagen IV molecule (basement membrane collagen) was identified. The distributions of collagen IV, laminin, and an additional extracellular molecule, the 2G2 antigen (2G2-Ag), were followed immunocytochemically during early w...

journal_title：Developmental biology

authors： Murray MA,Fessler LI,Palka J

更新日期：1995-03-01 00:00:00

abstract：:Eight series of mouse chimeras were produced by aggregating 8-cell embryos that differed at the Gpi-1s locus, encoding glucose phosphate isomerase (GPI-1). Chimeric blastocysts (Gpi-1sa/Gpi-1sa <--> Gpi-1sb/Gpi-1sb) were transferred to pseudopregnant females, which produced only GPI-1C enzyme. Quantitative electrophor...

journal_title：Developmental biology

authors： West JD,Flockhart JH,Keighren M

更新日期：1995-03-01 00:00:00

abstract：:The unfertilized oocyte contains various granules which serve as storage sites for proteins, the majority which are yolk granules or platelets. Yolk granules are thought to provide the developing embryo with nutrients essential for its survival, while other granules contain proteins such as enzymes and extracellular m...

journal_title：Developmental biology

authors： Reimer CL,Crawford BJ

更新日期：1995-02-01 00:00:00

abstract：:Previous studies from this laboratory have identified a novel alpha-D-mannosidase on the sperm plasma membranes of several species, including man, which may have a role in fertilization. The polyclonal antibody raised against an isoform of the enzyme purified from rat epididymal fluid was found to cross-react with the...

journal_title：Developmental biology

authors： Tulsiani DR,NagDas SK,Skudlarek MD,Orgebin-Crist MC

更新日期：1995-02-01 00:00:00

abstract：:In Hydra long-range interactions between head, bud, and foot formation take place in addition to short-range interactions which are recognized as induction phenomena, occurring when transplants are placed into an environment of disparate positional value. Here the long-range promotion of foot formation is analyzed. Th...

journal_title：Developmental biology

authors： Müller WA

更新日期：1995-01-01 00:00:00

abstract：:We report here the identification and characterization of a novel gene, T1 alpha, expressed in high abundance in adult rat lung, fetal lung, and early fetal brain. T1 alpha was identified by a monoclonal antibody previously shown to be specific for an antigen expressed by alveolar epithelial type I cells. The cDNA for...

journal_title：Developmental biology

authors： Rishi AK,Joyce-Brady M,Fisher J,Dobbs LG,Floros J,VanderSpek J,Brody JS,Williams MC

更新日期：1995-01-01 00:00:00

abstract：:Cortical granules of eggs contain a population of heterogeneous proteins that are sequestered selectively within the cortical granule during oogenesis and that participate in the block to polyspermy during the fertilization reaction. To begin to understand the targeting mechanism of proteins to this organelle, we have...

journal_title：Developmental biology

authors： Wessel GM

更新日期：1995-01-01 00:00:00

abstract：:Hox genes are thought to participate in patterning the anteroposterior (a-p) axis during vertebrate embryogenesis. In this investigation, the spatial expression of six Hoxb genes was analyzed in early embryos of Xenopus laevis by in situ hybridization. Hoxb gene expression was first detected in late gastrulae/early ne...

journal_title：Developmental biology

authors： Godsave S,Dekker EJ,Holling T,Pannese M,Boncinelli E,Durston A

更新日期：1994-12-01 00:00:00

abstract：:To investigate the mechanism by which the hormone 17 alpha,20 beta-dihydroxy-4-pregnen-3-one(17 alpha,20 beta-DP) acts on a receptor on the external surface of rainbow trout oocytes to induce maturation, the interaction between 17 alpha,20 beta-DP receptors and G-proteins was examined. Pertussis toxin (PT) catalyzed t...

journal_title：Developmental biology

authors： Yoshikuni M,Nagahama Y

更新日期：1994-12-01 00:00:00

abstract：:We have examined the response of bovine oocytes matured in vitro for 24 hr to parthenogenic activation using compounds that increase intracellular calcium (ionomycin) or inhibit protein phosphorylation (6-dimethylaminopurine, DMAP). Treatment with ionomycin alone caused resumption of meiosis (57.8 +/- 7.8%) but not pr...

journal_title：Developmental biology

authors： Susko-Parrish JL,Leibfried-Rutledge ML,Northey DL,Schutzkus V,First NL

更新日期：1994-12-01 00:00:00

abstract：:The expression of heat shock genes is induced in all living cells by a series of proteotoxic treatments. Heat shock genes are also activated spontaneously during different phases of embryonic development. HSP89 alpha and HSC70 are expressed at a high level in the mouse blastocyst. A family of factors, called HSFs, are...

journal_title：Developmental biology

authors： Mezger V,Rallu M,Morimoto RI,Morange M,Renard JP

更新日期：1994-12-01 00:00:00

abstract：:Exposure of Xenopus animal pole explants to transforming growth factor beta 2 (TGF-beta 2) induced the sequential expression of muscle nicotinic acetylcholine receptor (AChRs) isoforms and their corresponding mRNAs in cells which normally give rise to ectoderm. Single channel recordings revealed two functional classes...

journal_title：Developmental biology

authors： Reuer Q,Kullberg RW,Owens JL

更新日期：1994-11-01 00:00:00

abstract：:The finding that retinoic acid (RA) has major effects on pattern formation in developing chick limbs has led to the conclusion that RA plays a central role in the normal development of the limb. In addition, it has been demonstrated that treatment with exogenous RA leads to changes in expression of homeobox-containing...

journal_title：Developmental biology

authors： Hayamizu TF,Bryant SV

更新日期：1994-11-01 00:00:00

abstract：:Oct-4 is the earliest expressed gene known to encode a transcription factor which is developmentally regulated during mammalian embryogenesis. In order to understand the role of Oct-4 in early murine embryogenesis, we carried out an analysis of the temporal and spatial pattern of protein expression. We report the pres...

journal_title：Developmental biology

authors： Palmieri SL,Peter W,Hess H,Schöler HR

更新日期：1994-11-01 00:00:00

abstract：:Recombinant limb buds constructed of an ectodermal jacket containing dissociated anterior wing bud mesoderm cells form limb-like structures lacking anteroposterior polarity. Polarity can be restored by implanting a small piece of polarizing region into the recombinant limb. We used recombinant limbs to study expressio...

journal_title：Developmental biology

authors： Ros MA,Lyons GE,Mackem S,Fallon JF

更新日期：1994-11-01 00:00:00

abstract：:An abundant phosphoprotein with an apparent molecular mass of 65 kDa (p65) has been identified that is enriched in developing new macronuclei (or anlagen) isolated from the holotrichous ciliate, Tetrahymena thermophila. During early stages of macronuclear development, p65 is actively synthesized and deposited into you...

journal_title：Developmental biology

authors： Madireddi MT,Davis MC,Allis CD

更新日期：1994-10-01 00:00:00

abstract：:In the development of mammalian organs, a rapid and robust expansion of the parenchymal compartment must occur following allocation of organ primordia. This expansion must be regulated so that sufficient tissue mass is generated for further organization into functional tissues. The discovery that mosaic patches in the...

journal_title：Developmental biology

authors： Khokha MK,Landini G,Iannaccone PM

更新日期：1994-10-01 00:00:00